Use of the geneprintr 10 system with the applied biosystems. Mar, 2012 use of your genetic analyzer to determine the size of dna fragments making a multitude of fragmentbased applications possible on the same system as that for performing sanger sequencing. Applied biosystems 35003500xl genetic analyzer user guide 2010. Applied biosystems 35003500xl genetic analyzers support. Applied biosystems microamp 96well support base 10 bases life sciences.
Anjali, colin, safety,mark sweeny, environmental, health. See user s manual or package insert for limited label license, and trademark information. Google scholar an internal validation of the applied biosystems 8capillary 3500 genetic analyzer was performed using two commercial short tandem repeat str multiplex kits with focus on setting appropriate thresholds for data analysis. Perform the spectral calibration as described in the abi 3500 3500xl genetic analyzer user manual. Applied biosystems 35003500xl genetic analyzer user guide pn. Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 35003500xl genetic analyzer user guide part no. Anjali, colin, safety,mark sweeny, environmental, health and. The system features an advanced longlife solidstate laser, which reduces the physical and environmental footprint of the system as well as the cost of ownership. Applied biosystems 3500 genetic analyzer from thermo. Applied biosystems 3500 series genetic analyzers for dna.
User manual applied biosystems 3500xl user manual 326 pages. The 3500xl system uses a 24capillary array and singleline 505 nm, solidstate longlife laser for significantly improved signal uniformity. Boot pc, login windows as 3500 user, wait for server monitor green check mark in the. Applied biosystems 3500 hid genetic analyzer 3500xl. The complete product combines the instrument with applied b.
Dna sequencers genetic analyzers genetic analyzers are automated systems capable of sequencing dna or analyzing fragments for a variety of applications. With this interface, users can clearly monitor the state of. Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 35003500xl genetic analyzer user. Internal validation of the applied biosystems 3500xl genetic.
Advanced multiplex capabilities for dna fragment analysis containing up to six unique dyes. For additional training and reference information, see the user documents provided with the instrument. Technical data sheet leukostrat cdx flt3 mutation assay. They are all endoflife and endofsale starting in 2002. Applied biosystems 3500 or 3500xl genetic analyzers 3500 series instruments. The 3500xl genetic analyzer has increased the number of capillaries used, allowing more sample runs per injection while reducing the time of data output.
Details of purchase order of applied biosystems 3500xl. Your payment may be delayed if you do not follow the instructions noted below. Abi, summary of steps recommended order start instrument via power on button. Hid field applications specialist afdaa meeting austin, tx feb, 20. Applied biosystems 35003500xl genetic analyzer user. Browse our useful resources and support for your 3500 or 3500xl genetic analyzer including setup and maintenance as well as troubleshooting advice.
Estimating allele dropout probabilities by logistic. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument. Details as per annexure i 1,65,000 details as per annexure i total cip mumbai, price in usd one lac sixty five thousand only 1,65,000 important note. This validation was aimed to demonstrate that there is no effect on the applied biosystems 3500xl genetic analyzer thermo fisher scientific, uk after decommissioned and recommissioned at new premises. This instrument would be a fundamental component of the dna sequencing core facility, fulfilling the demanding research requirements that span multiple areas of study. Innogenomics technologies instruction manual igt 5dye matrix standard instruction manual version 3. Use of the msi analysis system with the applied biosystemsr. This phenomenon is characterised by a strong sample in an injection imparting lowlevel peaks to negative. Applied biosystems 35003500xl genetic analyzer user bulletin.
Upgrade of applied biosystems genetic analyzers, 3500 series. With this interface, users can clearly monitor the state of consumables and schedule maintenance protocols. Rfid system of 3500 series which performs automatic detection of reagents and instrument accessories used, forces the instrument operators to replace these reagents and accessories unnecessarily frequently, nonefficiently and makes the whole operation of 3500 series very expensive. Additionally, plate setup is simplified, as is built in primary analysis and quality control. Upon installation of a new 3500 series instrument for hid analysis, and prior to. This instrument would be a fundamental component of the dna sequencing core facility, fulfilling the demanding research requirements that. B genemapper id software applied biosystems 3 or 3xl genetic analyzer section 5. In capillary electrophoresisbased systems, dna fragments bound to probes migrate through a polymer and the fluorescence emissions are measured. Feb 17, 2010 the 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative consumables system approach.
Hence this instrument would be a jointuse instrument, used in a variety of projects across several investigative. The 3500 series genetic analyzers1 are specifically designed to support the demanding performance needs of validated and. The 3500 series gas are developed with features useful for forensic scientists, including a normalization feature for analysis of the data designed to reduce the variation in peak height. Two user attention words appear in applied b iosystems user. Page 1 applied biosystems 3500 3500xl genetic analyzer user guide with 3500 series data collection software 3. Manuals and user guides for applied biosystems 3500xl. View online or download applied biosystems 3500xl user manual. A genemapper idx software abi prism 3100 or 3100avant genetic analyzer section 5. Oct 12, 2011 applied biosystems, user bulletin applied biosystems 35003500xl genetic analyzer, foster city, ca, 2011. Applied biosystems 35003500xl genetic analyzer with. Applied biosystems 3500xl manuals manuals and user guides for applied biosystems 3500xl. Jan 23, 2012 working together, these elements provide the highest level of performance from a genetic analyzer to date.
Identifiler and identifiler plus thermal cycling protocols followed manufacturer recommendations found in each kits manual, and was carried out on the geneamp 9700 thermal cycler applied biosystems. Google scholar an internal validation of the applied biosystems 8capillary 3500. The 3500xl offers multiple advantages over the 3xl genetic analyzers that are being used at the mspftc. The majority of applications can be run on a single polymer and capillary array. Applied biosystems 35003500xl genetic analyzer site. We set a new standard in capillary electrophoresis with the 8capillary 3500 and 24capillary 3500xl genetic analyzers. Genetic analyzer 20 electrophoresis using the applied biosystems 33 xl genetic analyzer 28 electrophoresis using the applied biosystems 35003500xl genetic analyzer 38 analysis 50. The results showed genotyping concordance which means using. Powerplexr fusion system for use on the applied biosystems.
Applied biosystems 3500 and 3500xl genetic analyzers. Same sample sets were tested on the abi 3500xl genetic analyzer at both old and new premises using current accredited procedures. Page 1 applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. The 3500xl 24capillary platform is also fluorescencebased utilizing capillary electrophoresis to support for sequencing application and fragment analysis. Use of the msi analysis system with the applied biosystems. Applied biosystems 35003500xl genetic analyzers support thermo fisher scientific us. Multichannel pipettes, manual and electronic centrifuge capable of x g with a swingout rotor and refrigeration centrifuge capable of 1400 x g with a swingout rotor benchtop mini microcentrifuge.
Applied biosystems 3730 or 3730xl dna analyzers 3730 series. A new phenomenon was detected using the applied biosystems 3500xl genetic analyzer. The 3500 series data collection software integrates seamlessly with several downstream software packages to provide comprehensive analysis of genetic data. It can collect data in ivd mode for diagnostic hla typing, as. Use of your genetic analyzer to determine the size of dna fragments making a multitude of fragmentbased applications possible on the same system as that for performing sanger sequencing. Conditioning reagent for 3500 dx3500xl dx genetic analyzers. Lowlevel signal interference between samples separated on the 3500xl capillary electrophoresis platform article pdf available in australian journal of forensic sciences 483. Specification sheet 3500 and 3500xl series systems overview applied biosystems sets a new standard in capillary electrophoresis with the 8capillary 3500 and 24capillary 3500xl genetic analyzers. Applied biosystems 3500 3500xl genetic analyzer, 3500 series. The 3500xl genetic analyzer will be used for short tandem repeat str analysis because 3xl genetic analyzer has been discontinued. Pcr products were separated and detected on an abi 3500 genetic analyzer using a 36 cm array and pop4 polymer applied biosystems. The 3500 series genetic analyzers set a new standard in capillary electrophoresis, with increased throughput, faster run times, and an innovative. It can collect data in ivd mode for diagnostic hla typing, as well as ruo mode.
Sequencing throughput and performance specifications. Applied biosystems 35003500xl genetic analyzer user guide with 3500 series data collection software 3. Series genetic analyzer cathode buffer, anode buffer, and conditioning reagent are designed for readytouse, loadandrun installation. The 24capillary 3500xl genetic analyzer sets the standard in capillary electrophoresis. Applied biosystems 35003500xl genetic analyzer user guide applied biosystems, faster city ca. Offlabel uses such as fragment analysis are for research use only. Biosystems 35003500xl genetic analyzer user guide part no. The purchase price of this applied biosystems 33 xl genetic analyzer includes a grant of a. Guidelines for the validation process are published in the internal validation guide of. Applied biosystems 3500 genetic analyzer biosurplus. The 3500series instruments are the first genetic analyzers designed with a specific feature set and workflow for the.
Bob morrison stlcccpls, instrumentation specialist initiated. Data collection with the 3500 genetic analyzer is user friendly and intuitive, with simple dashboard navigation and easy to read displays. Applied biosystems, user bulletin applied biosystems 35003500xl genetic analyzer, foster city, ca, 2011. An overview of the powerplex fusion system protocol. Compared with the previous 3 xl genetic analyzer 3. Powerplexr 5c matrix standard technical manual tmd049. Applied biosystems 3500 or 3500xl genetic analyzer section 5. Dna fragment analysis by capillary electrophoresis user guide. Applied biosystems microamp 96well support base 10 bases. Applied biosystems 3500 genetic analyzer from thermo fisher. We have 1 applied biosystems 3500xl manual available for free pdf download. Audience this manual is written for principle investigators and laboratory staff who are. Abi maintenance, calibration, and supplies prepared by. The 3500series instruments are the first genetic analyzers designed with a specific feature set and workflow for the human identification application.
Details as per annexure i 1,65,000 details as per annexure i total cip mumbai, price in usd one lac sixty five thousand only 1,65,000 important. Also, perform any required maintenance tasks as indicated by the instrument gauges and maintenance notifications on the data collection dashboard screen described in the applied biosystems 3500 3500xl genetic analyzer user guide part no. For additional information on performing spectral calibration, refer to the applied biosystems 35003500xl genetic. Lowlevel signal interference between samples separated on. Dk ebook user manual guide referencenice ebook you should. Making second natureour first priorityit works the way that you work. The 3500xl dx genetic analyzer cs2 uses capillary electrophoresis and sanger technology to carry out accurate, reliable sequencing analyses.
847 786 709 426 293 491 1035 592 45 1213 1245 135 623 711 440 156 361 47 1500 750 190 139 636 755 1207 1360 1318 515 1078 681 576 1464 1036 165 1249 1397 1233 1201 538 192 234 913 1150 1392 4 672